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The inclusion criteria included the presence of diabetes mellitus with stable glycemic control as assessed by the patient's primary physician, signs of peripheral neuropathy not attributable to another cause, and three months or more of daily pain of at least moderate severity, the quality and location of which were consistent with the peripheral neuropathy ( Table 1).
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The transcriptional silencer ability of a polypeptide can be assayed by: 1) constructing an expression vector that encodes the test silencer polypeptide linked to another polypeptide having DNA binding activity (ie, constructing a DNA binding domain-silencer domain fusion protein), 2) cotransfecting this expression vector into host cells together with a reporter gene construct that is normally constitutively expressed in the host cell and also contains binding sites for the DNA binding domain and 3) determining the amount of transcription of the reporter gene construct that is inhibited by expression of the fusion protein in the host cell. 3 In lopinavir truvada cases, treatment is focused lopinavir harga evacuation of the gastric contents.
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The reaction is catalyzed with a palladium catalyst (eg, Pd(OAc) ).
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